Lentinula edodes (=Lentinus edodes) : Shiitake Mushroom Culture Slant

Our Price

$55.00

Weight 0.35 lbs
SKU CULTsl-060
Quantity
Description

Cultivation Difficulty: Moderate
Type: Edible/Medicinal
Substrate: hardwood chips and sawdust, hardwood logs
Colonization/Fruiting Temperatures: 70-80F/50-70F

Shiitake is a very popular edible mushroom that has been cultivated in Asia for centuries. It is a dense, chewy and meaty flavored mushroom that is good fried or grilled.

Shiitake reportedly has wide-ranging health promoting and immunity stimulating properties against cancer, viral infection and high cholesterol.

This particular strain (see picture) has spontaneously formed fruit bodies in 4 months on malt agar under refrigeration.

Fruiting formula by volume:

100 parts hard wood sawdust/small woodchips

10 parts bran

1 part gypsum (calcium sulfate)

Water until moist and well soaked through. Drain or squeeze out excess water. The substrate should be damp but not wet. Pack into autoclavable bags and sterilized from 1-4 hours at 15 psi depending on the volume of the substrate. Larger volumes need a longer sterilization time to heat the core enough for complete sterilization. Depending on the type of bag and method of inoculation, the bags can be sealed before or after sterilization. Colonization is slow and may take as long as 1-2 months.

Fruiting requires removing or puncturing the bag and providing a cool, well ventilated and humidified environment with indirect light. Hand misting daily and covering the bagless sawdust block loosely with a light plastic grocery bag (to maintain humidity) gives acceptable results on the kitchen counter.

Culture supplied as living mycelium in a test tube slant. Culture can be stored under refrigeration for several months. Slants are best suited for long distance travel, international shipments, or long term culture storage. To ensure freshness of culture, slant requests are made to order, please allow 2-4 weeks for processing and shipment.

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